[Study on Chemical Constituents of Fermented Antrodia camphorata Powder].

OBJECTIVE: To study the chemical constituents of fermented Antrodia camphorata powder. METHODS: 15 compounds were isolated from Antrodia camphorata by Silica gel column chromatography, ODS column chromatography, gel column chromatography, preparative liquid phase chromatography separation technique, as well as recrystallization. RESULTS: On the basis of their physical and chemical properties and spectral data,their structures were identified as Ferulic acid (1), Inositol (2), ß-Sitosterol (3),Vanillin (4),Vanillic acid (5), Butyric acid (6), Daucosterol (7), p-Hydroxycinnamic acid (8), Lauric acid (9), Inosine (10), Uridine (11), Adenine (12), D(+)-Sucrose (13), Arachidic acid (14) and Guanosine (15). CONCLUSION: Compounds 1, 5, 6 and 8-15 are isolated from fermented powder for the first time.

[Determination of inorganic elements in Antrodia camphorata powder by ICP-MS].

OBJECTIVE: To establish a method for simultaneous analysis of 22 inorganic elements in Antrodia camphorata powder by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Additionally, inorganic elements of Ganoderma lucidum had been detected and compared. METHODS: The sample solutions were analyzed by ICP-MS after microwave digestion and the contents of elements were calculated according to their calibration curves. The data of correlations and principal components were analyzed with the SPSS 17.0 software. RESULTS: 22 inorganic elements were determined in Antrodia camphorata powder. And there are some correlations among the inorganic elements in Antrodia camphorata powder. In addition to the element K, the content of Ca and Mg was also abundant. The content of heavy metals and harmful elements should be pay more attention. CONCLUSION: The study indicates that ICP-MS is a quick, accurate and sensitive method for determining the content of inorganic elements in Antrodia camphorata powder, which provides a scientific reference for the quality analysis and safety assessment of Antrodia camphorata powder.

[Analytical studies on flavonoids constituents in Apocynum venetom leaves by UPLC-Q-TOF-MS].

OBJECTIVE: To establish an ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometric (UPLC-Q-TOF-MS) method for analyzing the flavonoids constituents in Apocynum venetom leaves. METHODS: The analysis was performed on an Alltima C18 analytical column with a gradient solvent system of acetonitrile-1.0% acetic acid at the flow rate of 0.8 mL/min. The detection wavelength was at 254 nm and the column temperature was at 30 degrees C. Identification and detection were collected under the negative electrospray ion mode using Q-TOF-MS. RESULTS: 11 flavonoids constituents being separated and identified, six of them were detected simultaneously. The results showed that the six detected flavonoids constituents had good linearity between concentrations and peak area (r > 0.9996), and the average recoveries of the method were between 94.3% - 103.3%. CONCLUSION: The method is accurate, reproducible, and can be used for the quality control of Apocynum venetom leaves.

[Supercritical CO2 fluid extraction and component analysis of leaves oil from Taxus chinensis var. mairei].

OBJECTIVE: To research the optimal extraction process of supercritical CO2 extraction and analyze the component of the oil extracted from leaves of Taxus chinensis var. mairei. METHODS: Using the yield of leaves oil from Taxus chinensis var. mairei as the index, investigated the effect of the extraction pressure, extraction temperature and extraction time on the extracting-rate of leaves oil. The chemical composition of the extracted leaves oil was analyzed by derivatized GC-MS. RESULTS: The optimal parameters of the supercritical CO2 extraction of the oil extracted from leaves of Taxus chinensis var. mairei were determined: CO2 compressor pump frequency was 10 Hz, the extraction pressure was 25 MPa and the temperature of extraction was 45 degrees C, the extraction time was 120 min, the isolator I pressure was 8.0 MPa and the temperature of extraction was 40 degrees C, the isolator II pressure was 5.0 MPa and the temperature of extraction was 35 degrees C. The extracted leaves oil was derivatized with boron trifluoride-methanol complex. Thirty-three kinds of fatty acids were identified by GC-MS. CONCLUSION: The yield of leaves oils are different from Taxus chinensis var. mairei from 3 habitats. The yield of leaves oil from Donggang, Wuxi city is the highest, about 2.61%. The kinds of fatty acids with high amounts in leaves oil from Taxus chinensis var. mairei is identical in general, the kinds of fatty acids with low amounts in leaves oil from Taxus chinensis var. mairei have differences.

[Preliminary analysis of quality of Antrodia camphorata powder].

OBJECTIVE: To proceed a preliminary analysis of the quality of Antrodia camphorata powder. METHODS: The contents of water-soluble extract were detected according to the standards stated in Chinese Pharmacopoeia. UV-VIS was used to analyze total polysaccharide and triterpenoid. HPLC method was applied for the analysis of adenosine in Antrodia. camphorata. Besides, volatile compounds were analyzed by HSGC-MS. RESULTS: The contents of water-soluble extract (37.26% - 40.98%), total polysaccharide (5.45% - 8.08%), total triterpenoid (2.44% - 2.87%) and adenosine (0.0470% - 0.0604%) were obtained respectively. 49 volatile compounds were identified in Antrodia camphorata powder. CONCLUSION: The established method can be used for the quality control of Antrodia camphorata powder.